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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 66-72 doi: 10.15302/J-FASE-2018229

Abstract: It provides a potential strategy for breeding resistant pigs.transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenicThese results have paved the way for future production of marker-free transgenic resistant to PEDV and

Keywords： piglet diarrhea     RNAi     PEDV     TGEV     transgenic resistance breeding    

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Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Strategic Study of CAE 2002, Volume 4, Issue 8,   Pages 34-39

Abstract: It described advances of transgenic breeding of rapeseed (Brassica napus) in Oil Crops Institute, HunanThe. paper dealt with Bt-transgenic insect-resistant rapeseed line, transgenic sterile line, restorerline and their hybrid, transgenic high oleic acid content rapeseed line and transgenic high oil content

Keywords： rapeseed     transgenic     breeding    

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Breeding for the resistance to

Hongxiang MA, Xu ZHANG, Jinbao YAO, Shunhe CHENG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 3,   Pages 251-264 doi: 10.15302/J-FASE-2019262

Abstract: Breeding for FHB resistance in wheat is an effective way to control the disease.through standard breeding methods and widely applied in production or breeding programs.Diagnostic molecular markers for 1 have been applied in wheat breeding and breeding lines with improvedFHB resistance and desirable agronomic traits have been obtained.However, breeding for FHB resistance is a long-term task, new technologies are likely to increase the

Keywords： breeding     Fusarium head blight     wheat    

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Transgenic technologies in cassava for nutritional improvement and viral disease resistance: a key strategy

Maliwan NACONSIE,Peng ZHANG

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 4,   Pages 285-294 doi: 10.15302/J-FASE-2016119

Abstract: Genetic engineering provides promising approaches to improve nutritional value and increase resistanceengineering protein content by overexpression of nutritious storage proteins and improving cassava resistance

Keywords： Manihot esculenta     protein enhancement     RNA interference     transgenesis     virus resistance    

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Progress in breeding for resistance to Ug99 and other races of the stem rust fungus in CIMMYT wheat germplasm

Sridhar BHAVANI, David P. HODSON, Julio HUERTA-ESPINO, Mandeep S. RANDHAWA, Ravi P. SINGH

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 3,   Pages 210-224 doi: 10.15302/J-FASE-2019268

Abstract: Concerted research efforts have resulted in the identification of several new resistance (APR) genesand gene combinations for use in breeding.trait loci conferring stem rust resistance has progressed in the recent years, enhancing the durabilityof resistance.Effective gene stewardship and new generation breeding materials and cultivars that combine multiple

Keywords： adult plant resistance     black rust     race-specific resistance     Triticum aestivum    

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Current Research Status of Heterodera glycines Resistance and Its Implication on Soybean Breeding Review

Guiping Yan,Richard Baidoo

Engineering 2018, Volume 4, Issue 4,   Pages 534-541 doi: 10.1016/j.eng.2018.07.009

Abstract: With increasing reports of virulent SCN populations that are able to break the resistance within commonlyused sources, there is an increasing need to find new sources of resistance or to broaden the resistanceThis review summarizes recent findings about the genes controlling SCN resistance in soybean, and abouthow these genes interact to confer resistance against SCN in soybean.lines and cultivars in order to expedite conventional breeding programs.

Keywords： Soybean cyst nematode     Heterodera glycines     Resistance     Molecular breeding    

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regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1,   Pages 87-96 doi: 10.15302/J-FASE-2016087

Abstract: that the pMAR-CAG-FST-IRES-AcGFP1-polyA-MAR (pMAR-FST) vector had higher capacity to form monoclonal transgenicRemarkably, protein expression in transgenic cells with the pMAR-FST vector was significantly higherExogenous was expressed in all of the pMAR-FST transgenic mice at F , F and F .mice was significantly greater than in wild-type mice, with larger muscles in fore and hind limbs of transgenicmice. pMAR-FST transgenic mice were also found with more evenly distributed muscle bundles and thinner

Keywords： safety of transgenic     bicistron gene transfer body     transgenic mice     muscle development    

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Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Strategic Study of CAE 2016, Volume 18, Issue 3,   Pages 105-109 doi: 10.15302/J-SSCAE-2016.03.018

Abstract: the US Food and Drug Administration (FDA) approved the "all fish" growth hormone (gh) transgenicgenetically engineered livestock approved for human consumption and is considered as breakthrough of transgeniclivestock breeding research and livestock industrialization.The US FDA made this landmark decision after 20 years rigorous bio-safety assessment of the transgenicBoth the scientific research behind the creation of this transgenic product and the active participation

Keywords： transgenic     Atlantic salmon     industrialization     enlightenments    

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Breeding for pre-harvest sprouting resistance in bread wheat under rainfed conditions

Muhammad ZEESHAN, Waheed ARSHAD, Muhammad Imran KHAN, Shiraz ALI, Ali NAWAZ, Amina BATOOL, Muhammad TARIQ, Muhammad Imran AKRAM, Muhammad Amjad ALI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 2,   Pages 253-261 doi: 10.15302/J-FASE-2018218

Abstract: 10 cultivars and five advanced lines of spring bread wheat were evaluated for pre-harvest sprouting resistanceCultivars BARS-09, 09FJ17, Doukkala-12, NARC-09 and Ouassou-20 exhibited higher sprouting resistancesix susceptible and two resistant genotypes to assess the genetic behavior of pre-harvest sprouting resistanceThe combining ability (CA) demonstrated a higher proportion of additive genetic effects for sprouting resistanceDoukkala-12 and BARS-09 showed increased pre-harvest sprouting resistance in their F descendants.

Keywords： bread wheat     germination index     general combining ability     pre-harvest sprouting     specific combining ability    

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Comments on this special issue on “Wheat Genetics and Breeding”

Zhonghu HE, Xu LLU

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 3,   Pages 309-309 doi: 10.15302/J-FASE-2019264

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Highlights of special issue on “Wheat Genetics and Breeding”

Zhonghu HE, Zhendong ZHAO, Shunhe CHENG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 3,   Pages 207-209 doi: 10.15302/J-FASE-2019263

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Genomics and genetic breeding in aquatic animals: progress and prospects

Wenteng XU, Songlin CHEN

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 3,   Pages 305-318 doi: 10.15302/J-FASE-2017154

Abstract: influenced by these findings, such as accelerated generation shift in the seed industry and the process of breeding, trait-related marker/genes screening, as well as sex control, genome editing, and other molecular breeding

Keywords： aquaculture     genomics     genetic breeding     progress    

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: One potential method is to produce transgenic livestock that make modified milk containing high concentrationSubsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generated transgenicanalysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary gland of the transgenicOur results pave the way for making transgenic livestock to produce large quantities of rhBCHE.

Keywords： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLF BAC)     transgenic    

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Wheat research and breeding in the new era of a high-quality reference genome

Rudi APPELS

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 3,   Pages 225-232 doi: 10.15302/J-FASE-2019265

Abstract:

The publications of the International Wheat Genome Sequencing Consortium (IWGSC) released in August 2018 are reviewed and placed into the context of developments arising from the availability of the high-quality wheat genome assembly.

Keywords： assembly technology     molecular markers     polyploidy     transcript networks     wheat genome    

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Genetic study and molecular breeding for high phosphorus use efficiency in maize

Dongdong LI, Meng WANG, Xianyan KUANG, Wenxin LIU

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 4,   Pages 366-379 doi: 10.15302/J-FASE-2019278

Abstract: genetic architecture of crop PUE, mining related quantitative trait loci (QTL) and using molecular breedingBased on this, a breeding strategy applying genomic selection as the core, and integrating the existinggenetic information and molecular breeding techniques is proposed for breeding high PUE maize inbred

Keywords： maize     phosphorus use efficiency     quantitative trait loci     genetic study     molecular breeding     genomic selection    

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Title Author Date Type Operation

Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Journal Article

Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Journal Article

Breeding for the resistance to

Hongxiang MA, Xu ZHANG, Jinbao YAO, Shunhe CHENG

Journal Article

Transgenic technologies in cassava for nutritional improvement and viral disease resistance: a key strategy

Maliwan NACONSIE,Peng ZHANG

Journal Article

Progress in breeding for resistance to Ug99 and other races of the stem rust fungus in CIMMYT wheat germplasm

Sridhar BHAVANI, David P. HODSON, Julio HUERTA-ESPINO, Mandeep S. RANDHAWA, Ravi P. SINGH

Journal Article

Current Research Status of Heterodera glycines Resistance and Its Implication on Soybean Breeding

Guiping Yan,Richard Baidoo

Journal Article

regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Journal Article

Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Journal Article

Breeding for pre-harvest sprouting resistance in bread wheat under rainfed conditions

Muhammad ZEESHAN, Waheed ARSHAD, Muhammad Imran KHAN, Shiraz ALI, Ali NAWAZ, Amina BATOOL, Muhammad TARIQ, Muhammad Imran AKRAM, Muhammad Amjad ALI

Journal Article

Comments on this special issue on “Wheat Genetics and Breeding”

Zhonghu HE, Xu LLU

Journal Article

Highlights of special issue on “Wheat Genetics and Breeding”

Zhonghu HE, Zhendong ZHAO, Shunhe CHENG

Journal Article

Genomics and genetic breeding in aquatic animals: progress and prospects

Wenteng XU, Songlin CHEN

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

Wheat research and breeding in the new era of a high-quality reference genome

Rudi APPELS

Journal Article

Genetic study and molecular breeding for high phosphorus use efficiency in maize

Dongdong LI, Meng WANG, Xianyan KUANG, Wenxin LIU

Journal Article